Sunday, 7 July 2013

Dinosaur warm-bloodedness: The whole Dinosauria (non-avian), Part 1


The central hypothesis in this series of posts on the debate about whether the dinosaurs were warm-blooded or not is that actually some types of dinosaurs were more warm-blooded and others were less; i.e. that there were different degrees of warm-bloodedness among different parts of the dinosaur group.

This idea calls for an in-depth analysis of all the different subgroups in the Dinosauria, but first I want to give an account of some of the evidence that has been used to argue about all dinosaurs as one. (Note that I naturally exclude the birds here: we know they are all fully warm-blooded.) I hope this both serves as an introduction to the main lines of reasoning, and also gives you an idea of why it makes more sense to split the diverse dinosaurs into smaller groups rather than treating them all equal.

I will start off with evidence in favour of warm-bloodedness in the whole Dinosauria, then move to evidence against, and finish off with an argument that warm-bloodedness must have at least evolved in one dinosaur group: the one close to true birds.

Something you need to understand about how paleontologists gather evidence for such things as warm-bloodedness is that there is no way of measuring it directly from fossils. Recall from the post explainingthe different types of ‘wam-bloodedness’ that the term refers to animals with stable body temperature (homeotherms), internal heat source (endothermic), and/or a high basal metabolic rate, or BMR (tachymetabolic); usually ‘warm-blooded’ comprises all three in combination. It is not possible to measure the live body temperature or metabolic activity of a dead animal that was turned into solid rock millions of years ago. So, there is no way we can truly show conclusively that the dinosaurs fit into any of these categories. What we can do is look at other features – features we can tell from the fossils, and that we have reasons to suspect are related to warm- or cold-bloodedness. We do this by examining differences between the modern animals we know are warm-blooded (mammals and birds) and those we know are cold-blooded (the rest), thinking about which differences could be linked to warm-bloodedness and why, and finally seeing if we can find the same features in the fossils. In other words, all evidence here is indirect evidence. It is important to be aware of this, because it poses a strong, inherent limitation to the quality of the evidence; but then again, it is the best we can do!


Evidence for warm-bloodedness
1. The reason the idea of warm-blooded dinosaurs was proposed in the first place is actually not that palaeontologists realised that they were closely related to birds, as you might think. Warm-bloodedness was suggested already in the 1980s when the comparative anatomist Sir Richard Owen noticed that the dinosaurs had an upright (or parasagittal) limb posture, a feature today only seen in birds and mammals, the only living warm-bloods. The other land vertebrates typically have a sprawling posture, their limbs splaying out to the sides, the upper part being closer to horizontal.

The main types of limb posture of land-living vertebrates. Sprawling is seen in most amphibians and ‘reptiles’, erect in dinosaurs (including birds) and mammals, and the pillar-erect in rauisuchians, ancient relatives of crocodiles, but extinct today. Image from http://en.wikipedia.org/wiki/File:Sprawling_and_erect_hip_joints_-_horiz.png
 
Of course, it is not enough to base such a claim on pure correlation: there needs to be a link between a parasagittal limb posture and warm-bloodedness. (In scientific terms, there must be a causal relationship between the two features.) And there is! It might be a bit complicated though, but bear with me and I will try to guide you through.

Recall from the postexplaining aerobic and anaerobic metabolism that the aerobic variant is about twenty times more energy-efficient, but demands and consumes oxygen, while anaerobic metabolism works in oxygen-free conditions, but produces much less energy. Warm-blooded animals, which use a large proportion of the energy they gain from metabolic processes to convert into heat to warm their bodies from the inside, therefore require a metabolism that is predominantly aerobic. Otherwise, they would not gain enough energy from their metabolic conversion of food to fuel their internal heat machine.

Thus, warm-bloods must rely on a predominantly aerobic metabolism, and therefore need a large and constant oxygen supply, in order for their aerobic metabolism to work. If they do not get enough oxygen, they will need to resort to anaerobic metabolism to get the energy, but that will consume twenty times as much food, which will run out quickly. 

Warm-blooded animals then need an efficient respiratory system – i.e. a system to get oxygen from the air into the cells of their body.

Vertebrates have lungs, sacs of highly vascularised tissue attached to muscles around the ribs. The millions of tiny, thin blood vessels in the lungs make up a huge surface area for exchange of gases between the blood and the air inside the lungs. The blood that enters the lung vessels has passed through the body, delivering oxygen for the cells to maintain themselves and taking up carbon dioxide they leave as a waste product. Therefore, that blood has plenty of dissolved carbon dioxide but little oxygen. When it comes in contact with the air, which has relatively less carbon dioxide but more oxygen, both gases are exchanged. They move from where there is plenty to where there is less, in order to balance out between the two sites. In effect, the blood gets rid of some carbon dioxide and picks up essential oxygen.

However, after gas exchange, the air in the lungs has as much carbon dioxide and oxygen as the blood, so when new blood enters the lungs, they will already be balanced and no more exchange will take place! For that reason, we need to replace the air in the lungs with fresh air from the outside. We call this ventilation, or breathing; for that, we use the ribs.

The rib muscles perform cycles of contractions to alternately expand and compress the torso, and, in effect, the lungs as well. When the lungs expand, the air pressure inside becomes lower than the pressure of the air outside. This pressure difference causes air to flow from the outside into the lungs. (Air always flows from a region of high air pressure to where the pressure is relatively lower; this is what causes wind, for example.) When the lungs contract, they force out some air from the lungs. This is how we get fresh air in and used air out. Without ventilation, gas exchange will not occur, and we will quickly starve our oxygen supplies, and, as a result, not be able to carry out aerobic metabolism.

Now you must be wondering what this has to do with having straight or bent legs. I will come to that just now! The thing is, except those with a parasagittal limb arrangement, all vertebrates move forward primarily by waving their bodies sideways. We all know that fish swim that way, and that set a precedent for their close land-living descendants. Maybe you have not thought about it, but most amphibians and reptiles in general move this way as well. I will not go into detail on their biomechanics (mostly because my understanding is not great), but, basically, their limbs are not flexible enough to reach far forward and backward, so their strides would be very short if they only used the limbs. By also turning their limb girdles, they achieve a considerably longer stride length, allowing fairly efficient locomotion.

This mode of moving is not only a residue from their fish ancestors, but is kept also in advanced amphibians and reptiles that have lost their limbs, e.g. caecilians (a very worm-like type of amphibians) and snakes. Exceptions to this include frogs (which jump rather than stride) and chameleons (which actually have more of a parasagittal limb posture than most reptiles, but this is because they are fairly large lizards moving around on rather narrow tree branches, so they need to have their limbs straight in order to achieve sufficient… narrowness to get a decent grip). However, these are special cases; they do not have a wave-like body motion for special reasons, unlikely to be related to their metabolism.

The importance of this locomotion style has to do with how they turn their limb girdles: the muscles on the sides of the body, between the front and hind legs, contract and relax alternatingly. When the trunk muscles on the left side contract, the left hind foot is moved forward, closer to the left front foot, and the right front foot is moved forward, away from the right hind foot. The next step is to let the trunk muscles on the right side contract, while those on the left relax. If the animal keeps the left hind foot and right front foot still in the ground, the remaining two feet will be moved forward, this time taking the left front foot further away from the left hind foot, and bringing the right hind foot closer to the right front foot. The animal moves forward by repeating these steps again and again, in a cycle.

I hope that made sense, but if not, do not be alarmed, it is not essential to grasp this. Actually, if you just look up some videos of walking reptiles on YouTube, I’m sure it will become much clearer. Regardless, the key thing to note is that half of the body contracts with every step. This contraction compresses the lung on that side of the body, forcing air out. 

Wow! So they breathe by moving!... You might think so, but that is a hasty conclusion. While one lung is compressed, the other is not expanded (I think… might need to check this up, actually…), so air is not sucked in there. And, even if it did, it would still mean that they only use half their lung capacity while moving. This puts a severe restriction on their maximum oxygen intake. Since oxygen is needed for aerobic food conversion, their ability to perform this is equally limited. As a result, most amphibians and reptiles become completely exhausted after a few minutes of strenuous activity, because anaerobic food conversion is so inefficient.

Now, this means that these sprawling-gaited animals are not very active animals. Because their energy production is so inefficient while they are moving, they spend most of their days resting. (In general, they are active for only a few hours every day, during which they forage for food, carry out domestic chores, etc.)

Now, here is where the argument might get quite confusing: because they are not particularly active, they would not gain much from having an active metabolism (i.e. having a high BMR; tachymetabolic). An active metabolism here means a metabolism that is predominantly aerobic – i.e. the bulk of the food is converted into energy aerobically. As explained in the previouspost on this topic, such a metabolism provides stamina in the form of a fairly high and continuous energy production, while the alternative – a predominantly anaerobic metabolism – normally has low energy output, but is capable of mobilising vast amounts of energy very rapidly, thus granting impressive burst speed. As a result, predominantly aerobic animals can be active for longer periods of time. That is the main advantage of such a metabolic strategy, but the sprawlers would still be restricted on this very aspect due to their less efficient walking style. They would gain little from having an aerobic metabolism, as the advantage it would grant would still have been limited due to their poor oxygen intake.

The advantage of aerobic metabolism is manifested when the animal is active. But this is also when the oxygen intake of amphibians and reptiles is as lowest, meaning that they will not be able to rely on an aerobic metabolism during activity. In other words, their particular gait thwarts the aerobic processes at the precise point where they would have been effective.

The disadvantage of a predominantly aerobic metabolism is that the metabolic rates are also elevated at rest. This means they consume more food during physical inactivity compared to animals relying on an anaerobic metabolism, which have very low rates of energy production (and thus also energy usage) during rest. Having a high energy production while not using any energy is simply a waste, as there is no gain, only higher costs of food.

To summarise, the way most amphibians and reptiles move severely limits their oxygen intake by compressing one of their lungs with every step. If they had been warm-blooded – i.e. had an active metabolism – this problem would have thwarted the advantage of stamina during activity. With the main advantage removed, the only effect of having such a metabolism would be suffering the main disadvantage of higher food consumption while at rest. 

This is the reason why a sprawling limb posture is associated with cold-bloodedness. Conversely, it is also the reason why a parasagittal limb posture is thought to have a connection with warm-bloodedness. Having the limbs straight under the body allows them to swing forward and backward comfortably using only muscles from the limbs and girdles. No other parts of their body need to contract or expand during such movement, so no lungs are compressed; they can breathe to their full capacity even while running, and, as a result, an aerobic metabolism would prove advantageous for them!

Now, a keen critical thinker would realise that the argument that dinosaurs were warm-blooded because they had a parasagittal limb arrangement is based on the assumption that dinosaurs would have evolved warm-bloodedness simply because it would have granted them an advantage. This is just as valid as arguing that ancient apes would have evolved brains of size and complexity rivalling that of humans because it would have granted them an advantage. Clearly, that did not happen.

Evolution just does not work like that. A human-sized brain is arguably an advantage to any animal (except sessile or planktonic filter-feeders… but let us not go there now…), but it is only us, among all the billions of species of animals that have ever evolved, that successfully achieved such cognitive complexity. Why? Because we had the genes for it! Without the necessary genetic material, the trait cannot develop in the first place.

This is why one cannot assume that dinosaurs would have developed full warm-bloodedness just because it would have been advantageous, not without having any reason to suspect they had the necessary genetic background. The fact that birds are warm-blooded gives us a clue that this genetic background at least evolved somewhere within the dinosaur group, but perhaps not necessarily at the base of it, i.e. not necessarily at the same time as they evolved a parasagittal limb posture.

The only difference these limb posture variants make is that if any sprawling-gaited amphibian or reptile had acquired the necessary genetic material to achieve full warm-bloodedness, their limb posture means they would not have gained any advantage from it, but rather a big disadvantage, and so it would not have become prominent in these groups. Dinosaurs, on the other hand, had the potential to gain an advantage from being warm-blooded. That is all.

Dear God, this discussion got far longer than I would have expected. I wanted to keep it brief and simple. But, my desire to explain it from the most basic level made it necessary to describe all parts of the argument thoroughly. Therefore, I decided to actually cut here, or the post would be dauntingly long. The remainder of the arguments for dinosaur warm-bloodedness will be the topic of Part 2.  

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